Application

human heart function, neointimal formation, vascular remodeling, myocardial repair, extracellular maintenance, signaling, toxicology, gene expression, structural support for cardiomycytes, synthesis of extracellular matrix, growth factors and cytokines

Cell Line Origin

Heart

Components

Fibroblast Basal Medium that contains 10% FBS and 10% DMSO

General description

Cardiac fibroblasts are the most prevalent cell type in the heart, making up 60-70 % of all cells. They are known to play key roles in the development of cardiac disease and hypertrophy. Rat Cardiac Fibroblasts provide an excellent model system to study many aspects of heart function and pathophysiology, including the regulation of cell growth, survival, inflammation, hormone secretion and metabolism.

Preparation Note

2nd passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO Can be cultured at least 8 population doublings

Subculture Routine

Please refer to the RCF Culture Protocol.